Two electrode voltage clamp electrophysiology (TEVC) is a commonly used electrophysiology method for oocyte recording, and npi electonic’s TEC is the best known amplifier for this purpose because of its precise measurement of ionic current flow across cell membranes. In this video, Dr. Dempski from Worcester Polytechnic Institute shows a powerful method to study the conformational changes of membrane proteins in situ by simultaneously monitoring fluorescence and current changes using voltage-clamp fluorometry with npi’s TEC amplifier. This method also allows investigators to manipulate intracellular and extracellular environments including ion composition, pH, and ligands to study membrane protein dynamics.
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Epidural cortical signals were detected with npi electronic’s differential amplifier EXT 10-2F to study the relationships between the calcium signals and the electrical activity. This configuration made it possible to record global ‘up states’ of the epidural potentials, although not the local electrical responses near the fiber.
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npi electronic’s EXT-02F is widely used for in vivo recording of C-fiber-evoked field potentials through stimulation of the sciatic nerve using npi’s ISO-STIM 01D. Results show the functional role of dura mast cells in nociception and synaptic plasticity in the superficial spinal dorsal horn.
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Breakthrough methods for in vivo electrophysiology now allow scientists to perform whole-cell recording of neurons in freely moving rats. Npi electronic’s ELC amplifier and mini-headstage have made it possible for this advanced research. The results from these recordings provide a better understanding into the mechanisms underlying neural activity during natural behaviors.
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Epidural cortical signals were detected with npi electronic’s EXT 10-2F differential amplifier to study relationships between calcium signals and electrical activity. Two-photon calcium imaging followed by optical fiber recordings make it possible to record global ‘up states’ of the epidural potentials.
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Wide-field ratiometric fluorescence imaging combined with in vivo recordings of local field, using npi electronic’s ELC-03XS, allows for longitudinal studies that optical imaging methods limit. It is now possible to study the long term stability and plasticity of cortical sensory representations.
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